Fig. 7: Activation of viral promoter PSCBV-YZ2060 by transcription factors ScbZIP72 from sugarcane and AREB1 from Arabidopsis. | Communications Biology

Fig. 7: Activation of viral promoter PSCBV-YZ2060 by transcription factors ScbZIP72 from sugarcane and AREB1 from Arabidopsis.

From: Identification of a sugarcane bacilliform virus promoter that is activated by drought stress in plants

Fig. 7

a Schematic map of the effector and reporter constructs: transcription factors ScbZIP72 from sugarcane or AREB1 from Arabidopsis in plasmid vectors Ubi:ScbZIP72 and Ubi:AREB1, Pmini35S:GUS vector, P4×sL-mini35S:GUS = four tandem repetitions of AREB1 (sL) in Pmini35S:GUS vector, and P4×msL-mini35S:GUS = msL (mutated sL) in Pmini35S:GUS. b Yeast one-hybrid assay with different combinations between ABRE and ScbZIP72 or AREB1: sL-AbAi/pGADT7-AREB1 = ABRE motif sL + AREB1; sL-AbAi/pGADT7-ScbZIP72 = ABRE motif sL + ScbZIP72; msL-AbAi/pGADT7-AREB1 = ABRE mutated motif msL + AREB1; msL-AbAi/pGADT7-ScbZIP72 = ABRE mutated motif msL + ScbZIP72; sL-AbAi/pGADT7 = negative control; p53-AbAi/pGADT7-p53 = positive control. c EMSA showing that ScbZIP72 can directly target sL by binding to the ABRE motif. d GUS protein expression in Arabidopsis protoplasts of ABRE motif 4xsL of promoter PSCBV-YZ2060 and mutants of this promoter motif after 10 μM ABA treatment (6 h) or co-transformation with bZIP transcription factors AREB1 and ScbZIP72. Control = no treatment. e GUS protein expression in Arabidopsis protoplasts of promoter PSCBV-YZ2060 and mutants of this promoter after co-transformation with bZIP plant transcription factors (AREB1 or ScbZIP72). Vector control = Ubi1:GUS. Values are the means (±standard errors) of four biological replicates and three technical replicates for each experiment. For each assay and for each vector or promoter, mean values with the same letter are not significantly different at P = 0.05 according to Duncan’s multiple range test.

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