Fig. 4: Effect of submergence on Rorippa aquatica leaf morphology and gene expression.

a Images and outlines of newly emerged young leaves after transfer to terrestrial or submerged conditions (scale bar, 1 cm). b Transcriptome analysis of leaves grown under the submerged condition. Differentially Expressed Genes (DEGs) were identified based on significant differences (FDR < 0.01) in expression and log2(fold change) |Log2FC| > 1. Based on their expression patterns, DEGs were categorized as “early response genes” (responding only within the first hour), “late-response genes” (after 4 days), and “throughout-response genes” (throughout submergence), then subjected to Gene Ontology enrichment analysis (significantly enriched categories are shown).