Fig. 2: CircTMCO3 was upregulated and M2 macrophage-derived exosomes could enter ovarian cancer cells. | Communications Biology

Fig. 2: CircTMCO3 was upregulated and M2 macrophage-derived exosomes could enter ovarian cancer cells.

From: M2 macrophage-derived exosomal circTMCO3 acts through miR-515-5p and ITGA8 to enhance malignancy in ovarian cancer

Fig. 2

a CircTMCO3 expression was determined by RT-qPCR in patient tissues. b Patient survival. c RT-qPCR analysis of circTMCO3 in A2780 and SKOV3 cells in coculture assays (n = 3). d Genomic loci and the junction site of circTMCO3. Sanger sequencing was applied to confirm the junction site. e, f The abundance of circTMCO3 and linear TMCO3 mRNA in SKOV3 in response to actinomycin D and RNase R treatment was determined through RT-qPCR (n = 3). g The abundance of circTMCO3, U6 and GAPDH in nuclear and cytoplasmic fractions from SKOV3 cells was examined by RT-qPCR (n = 3). h The examination of M2 macrophage-derived exosomes using TEM. Scale bar: 200 nm. i Exosome size was evaluated by NTA. j Protein levels of CD63, CD9, CD81 and TSG101 in M2 macrophages and exosomes were analyzed by western blotting. k Exosomes (Red) were labeled with PKH26 and endocytosed by A2780 and SKOV3 cells (Nuclei, blue) after 12, 24 and 48 h. Scale bar: 25 μm. *P < 0.05, **P < 0.01 and ***P < 0.001. Data were presented as mean ± standard deviation.

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