Fig. 6: miR-515-5p attenuated malignancy in ovarian cancer via downregulating ITGA8. | Communications Biology

Fig. 6: miR-515-5p attenuated malignancy in ovarian cancer via downregulating ITGA8.

From: M2 macrophage-derived exosomal circTMCO3 acts through miR-515-5p and ITGA8 to enhance malignancy in ovarian cancer

Fig. 6

a RT-qPCR analysis of ITGA8 in A2780 and SKOV3 cells transfected with OE-NC and OE-ITGA8 (n = 3). A2780 and SKOV3 cells were transfected with mimics NC, miR-515-5p mimics, miR-515-5p mimics in combination with OE-NC or OE-ITGA8. b, c RT-qPCR and western blotting analysis of ITGA8 (n = 3). d EdU incorporation analysis (n = 3). Scale bar: 100 μm. e Wound healing assays (n = 3). Scale bar: 250 μm. f Transwell assays (n = 3). Scale bar: 50 μm. g Protein levels of MMP9, E-cadherin, Vimentin and Snail were detected through western blotting. *P < 0.05, **P < 0.01 and ***P < 0.001. Data were presented as mean ± standard deviation.

Back to article page