Fig. 1: ROCK2 is upregulated in FSGS and in ADR-stimulated podocytes. | Communications Biology

Fig. 1: ROCK2 is upregulated in FSGS and in ADR-stimulated podocytes.

From: Deletion of podocyte Rho-associated, coiled-coil-containing protein kinase 2 protects mice from focal segmental glomerulosclerosis

Fig. 1

Renal ROCK2 distribution in human (a) and mouse (b). Single-cell RNA sequencing data were acquired from the Kidney Interactive Transcriptomics database (https://humphreyslab.com/SingleCell). Pod, podocyte; MC, mesangium; EC, endothelial cells; PT, proximal tubules; LOH (AL), loop of Henle, ascending limb; DCT, distal convoluted tubules; CNT, connecting tubules; PC, principal cells; IC-A, type A intercalated cells; IC-B, type B intercalated cells; tSNE, t-distributed stochastic neighbor embedding. c A correlation analysis between glomerular ROCK2 mRNA and urinary protein excretion in patients with FSGS. Hodgin FSGS Glom data obtained from the transcriptomic database Nephroseq (https://www.nephroseq.com) were used. The trendline is shown in red. The R-value was determined by Pearson correlation analysis (n = 6). d ROCK2 protein abundance in the renal cortex of ADR-injected mice (n = 3). e Representative immunostaining of ROCK2 (green) and nephrin (red) in the glomerulus of ADR-injected mice. Nuclei were visualized with DAPI (blue). The scale bar on the top left represents 10 μm. f The transcript levels of ROCK2 in glomeruli isolated from ADR-injected mice (n = 3). g The ROCK2 mRNA levels after ADR stimulation in cultured podocytes (n = 4). h ROCK2 protein levels in ADR-treated podocytes (n = 3). *p < 0.05. Data represent the mean ± s.e.m.

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