Fig. 2: cfCRISPR is a genome-wide cfDNA CRISPR-Cas9 screen that identifies putative modulators of cfDNA release. | Communications Biology

Fig. 2: cfCRISPR is a genome-wide cfDNA CRISPR-Cas9 screen that identifies putative modulators of cfDNA release.

From: An in vitro CRISPR screen of cell-free DNA identifies apoptosis as the primary mediator of cell-free DNA release

Fig. 2

a Fragmentation pattern of MCF-10A, healthy human donor control, and spike-in cfDNAs. Representative traces shown. b Relative representation of PIK3CA E545K and ERBB2 L755S mutations in cfDNA from isogenically modified double mutant MCF-10As and MCF-7s. Ratio of mutant droplets was detected and quantified by ddPCR to determine relative representation of each locus. n = 3 biologically independent samples c Overview of the cell-free DNA CRISPR Screen methodology. d Genes plotted by their β-scores in both cfDNA and gDNA arms of MCF-10A CRISPR screen. Putative hits are highlighted and grouped by shared function (green, apoptosis; pink, RNA binding; yellow, unknown). e Gene ontology of genes from the MCF-10A CRIPSR screen determined as putative hits. Data are −log p values derived from PANTHER gene ontology and include enriched pathways in biological process, molecular function, and cellular component.

Back to article page