Fig. 6: PAV-104 blocks SARS-CoV-2 virus-like particle assembly/budding.
From: A viral assembly inhibitor blocks SARS-CoV-2 replication in airway epithelial cells
a Western blot analysis of structural protein expression in cell lysates and ultracentrifuged pellets. HEK293T cells were transfected with plasmids encoding the proteins indicated at the top. Western blots were performed with the primary antibodies indicated on the left of the blots. Anti-β-actin antibody was used as a loading control. b, c Relative quantification of the indicated protein from western blot (a). β-actin was used as a loading control for cell lysates and pellets. The loading control was measured on the same blot (after stripping) alongside the other proteins in the experiment. d Quantification of SARS-CoV-2 VLPs by nanoparticle tracking analysis. HEK293T cells were transfected with plasmids encoding the proteins indicated at the top. VLPs containing nanoparticles in the ultracentrifuged pellets from cell culture supernatants were diluted to a concentration in the range of 107–109/ml and examined using a NanoSight NS300 (NanoSight, Ltd) equipped with a 405 nm laser. n = 5 biologically independent samples. Data are representative of the results as mean ± SEM. Statistical significance was analyzed by t test. p ≤ 0.05 [*], p ≤ 0.01 [**], p ≤ 0.001 [***], p ≤ 0.0001 [****].
