Fig. 4: Pocillopora verrucosa microbiome restructuring after long-term probiotic inoculation (T3 sampling time). | Communications Biology

Fig. 4: Pocillopora verrucosa microbiome restructuring after long-term probiotic inoculation (T3 sampling time).

From: Probiotics reshape the coral microbiome in situ without detectable off-target effects in the surrounding environment

Fig. 4

a Families among the top ten most abundant in the coral microbiome, with significant changes in relative abundance between placebo- and probiotic-treated corals. The statistically significant differences are denoted with asterisks: ∗∗p < 0.01; ∗∗∗p < 0.001. The depicted boxplots show the median (center line) and the first and third quartiles (lower and upper bounds). b Volcano plot depicting differentially abundant ASVs (dots) identified in the ANCOM-BC2 analysis (n = 1175 differentially abundant ASVs in probiotic- in comparison to the placebo-treated). The log fold change (X-axis) and the p-adj. (Y-axis) value for each ASV is represented. Blue dots indicate enriched ASVs and red dots indicate decreased ASVs with a q value (adjusted p value) < 0.05. ASVs that are not significantly different in abundance between treatments (probiotic vs placebo) are colored in gray. c Top differentially abundant ASVs with their associated genus taxa and color-coded by phylum. The W test statistic from the ANCOM-BC2 is shown (negative W indicates decreased taxa while positive W indicates enriched taxa in probiotic-treated corals in comparison to placebo-treated corals. “Unclassified” ASVs are available in Supplementary Table 3. d Differentially abundant ASV affiliated with the Vibrionaceae family, colored by genera. The dot size represents the Log Fold change of each ASV. Biological replicates per treatment (n = 15).

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