Fig. 3: Spectral signatures of β-carotene in BBP. | Communications Biology

Fig. 3: Spectral signatures of β-carotene in BBP.

From: Insights into the molecular mechanism of yellow cuticle coloration by a chitin-binding carotenoprotein in gregarious locusts

Fig. 3

a HPLC analysis showing that β-carotene is the predominant carotenoid associated with BBP purified from β-carotene-synthesizing E. coli cells. b Absorbance spectra of HPLC-purified β-carotene in various organic solvents as compared with the absorbance spectrum of β-carotene in BBP. Positions of the main maximum are indicated in parentheses in nm. Note the more pronounced vibronic structure of the BBP(βCar) spectrum. c UV/Vis circular dichroism spectra of β-carotene in n-hexane and in BBP. The UV/Vis circular dichroism spectrum of the BmCBP(ZEA) complex is shown for comparison. d The appearance of an aqueous 200 μM β-carotene solution in complex with BBP.

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