Table 2 Viral load in the selected tissues of dolphin

From: Highly pathogenic avian influenza A(H5N1) virus in a common bottlenose dolphin (Tursiops truncatus) in Florida

Dolphin’s tissue

Influenza A virus (M primers)

A(H5N1) virus (H5 primers)

Brain

15.7

23.6

Spinal Cord

22.0

26.1

Lung

27.8

32.5

Liver

34.3

–

Kidney

36.2

–

Gastric

28.9

34.2

  1. rRT-PCR CT values of influenza A matrix and H5 RNA isolated from dolphin tissues. RNA extracted from brain, spinal cord, lung, liver, kidney, and gastric samples was screened using influenza A virus M- and H5-specific primers and probes to test for the presence or absence of HPAI A(H5N1) virus by rRT-PCR. The CT value of the highest dilution of the controls was determined to be the limit of detection and served as the threshold for determining whether samples were PCR positive or negative. Numbers represent the CT values of selected tissues determined by rRT-PCR.
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