Fig. 1: Biosynthesis of H₂S was improved under cellulose culture condition.

a, b Change in the H₂S concentration was measured by SF7-AM fluorescence probe in wild-type (wt) strain under glucose or cellulose culture condition. The average fluorescence intensity values of all mycelia in the 6 photos were quantified. Scale bar = 100 μm. c Log₂(foldchange) of genes expression level of putative H₂S biosynthetic enzymes: L-cysteine desulfhydrase (lcd1 and lcd2), cysteine synthase (cs1 and cs2), cystathionine γ-lyase (cse1, cse2, cse3 and cse4), cystathionine β-synthase (cbs), 3-mercaptopyruvate sulfurtransferase (3-mst). The different letters indicate significant differences between the lines (“*“ means p < 0.05, “**“ means p < 0.01, “***“ means p < 0.001, according to Student’s t test). d, e Change in the H₂S concentration was measured by SF7-AM fluorescence staining in wt, sicontrol, cbs-silenced and cbs-overexpressed strains under glucose and cellulose culture condition. Scale bar = 100 μm. f Relative expression level of cbs gene in wt, sicontrol, cbs-silenced and cbs-overexpressed strains, cultured under glucose and cellulose condition. The different letters indicate significant differences between the lines (p < 0.05, according to Duncan’s multiple range test).