Fig. 4: CreA inhibits cbs transcription and reduces H2S biosynthesis. | Communications Biology

Fig. 4: CreA inhibits cbs transcription and reduces H2S biosynthesis.

From: The CBS/H2S signalling pathway regulated by the carbon repressor CreA promotes cellulose utilization in Ganoderma lucidum

Fig. 4

a, b Immunoblot analysis of CreA proteins in wt, sicontrol, creA-silenced and creA-overexpressed strains, cultured on CYM medium for 7 days. c Relative expression level of cbs gene in wt, sicontrol, creA-silenced and creA-overexpressed strains, cultured on CYM medium for 7 days. d, e Change in the H2S concentration was measured by SF7-AM fluorescence staining in wt, sicontrol, creA-silenced and creA-overexpressed strains, cultured on CYM medium for 7 days, measured by SF7-AM. Scale bar = 100 μm. The different letters indicate significant differences between the lines (“**“ means p < 0.01, “***“ means p < 0.001, according to Student’s t test).

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