Fig. 1: Blood exchange identifies differences in clearance kinetics of circulating tumor cells between leukemia and solid tumor models.
From: Leukemia circulation kinetics revealed through blood exchange method
a Overview schematic of blood exchange setup. A donor and recipient mouse have their circulation connected for 30 minutes to 1 hour. Following this blood exchange period, the mice are disconnected and the circulation from the recipient mouse is monitored for three hours. The blood passes through a laser on a microfluidic chip, and emitted light from fluorescent CLCs is detected by a photomultiplier tube (PMT). The raw data from the PMT is then processed to identify the concentration of CLCs, and the decay profile is used to extract circulation parameters. b Half-life times of circulating leukemia cells (AML and ALL) is several orders of magnitude higher than historical measures of various solid tumor circulating tumor cells12. SCLC small-cell lung cancer, PDAC pancreatic ductal adenocarcinoma, NSCLC non-small-cell lung cancer. Each dot represents an independent donor-recipient mouse pair.
