Fig. 3: Blood exchange identifies differences in clearance kinetics in healthy mice with either untreated or relapse donors.

a Decay profiles of untreated and relapse ALL CLCs in healthy recipient mice. b Relapse and untreated ALL CLCs have similar kinetics as measured by equilibration time (two-tailed t test p = 0.3257). c Decay profiles of untreated and relapse AML CLCs in healthy recipient mice. d Equilibration time of relapse AML CLCs significantly faster than untreated (two-tailed t test p = 0.0230). e Schematic of E-selectin adhesion to endothelial cells and E-selectin binding assay using fluorescent E-selectin. f E-selectin binding capacity increases in blood and marrow at relapse in AML and ALL (Tukey multiple comparisons: ***p < 0.001, ns p > 0.5). For decay profiles (a, c), shaded regions are represented by mean +/− standard error, and lines represent the best fit decay curve. For equilibration time analysis (b, d), each dot represents an independent donor-recipient mouse pair.