Fig. 1: DSF inhibited the B cell response more potently than any other known inhibitors related to B cell function. | Communications Biology

Fig. 1: DSF inhibited the B cell response more potently than any other known inhibitors related to B cell function.

From: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism

Fig. 1

a B-cell cluster formation after stimulation with IL4+anti-CD40 antibody as T-cell-dependent stimuli and LPS as T-cell-independent stimuli. b Number of B220 + B cells and CD138+ plasma cells recovered after 4 days of culture in the presence of DSF (NC, unstimulated, n = 5; stimulator, n = 5; DSF, n = 3 biologically independent samples). c IC50 values (nM) of DSF and DSF-related compounds (cyanamide, DDC and Cu(DDC)2), CCR2 or CCR5 inhibitors (INCB3344 and maraviroc, respectively), and known inhibitors for B cell activation (PI3K inhibitor LY294002 and wortmannin, BTK inhibitor Ibrutinib, NF-κB inhibitor Bay11-7082), for the inhibitory effect evaluated by the number of total B cells and plasma cells recovered after 4-day culture with LPS or IL-4+anti-CD40 antibody stimulation. d Number of EdU+ B cells recovered after 4 days of culture with IL4+anti-CD40 or LPS in the presence of DSF (NC, n = 6; stimulator, n = 6; DSF, n = 3 independent experiments). Significance was calculated using one-way ANOVA with Tukey’s post-hoc test: **P < 0.01, ****P < 0.0001. Data are shown as the mean ±  SEM.

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