Fig. 2: DSF relieving antibodies mediated rejection in murine cardiac transplantation. | Communications Biology

Fig. 2: DSF relieving antibodies mediated rejection in murine cardiac transplantation.

From: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism

Fig. 2

a Experimental design. b Survival curves of grafts. Syngeneic heart transplantation (Syngenic Tx, blue line, n = 10 biologically independent mice), allogenic heart transplantation (Allogenic Tx, red line, n = 10 biologically independent mice), and allogeneic heart transplantation with CD8+ cell depletion combined with the control diet (Cont, gray line, n = 10 biologically independent mice) or DSF diet (DSF, yellow line, n = 7 biologically independent mice). c Graft weight in the naïve group (n = 6 biologically independent mice), CD8-depleted group (Cont, n = 16 biologically independent mice and CD8-depleted with DSF-treated group (DSF, n = 17 biologically independent mice) postoperative day 9 (POD9). d Picture of grafts harvested at POD9. e–p Hematoxylin-eosin (e, f, g, h, i, j, k, m) or Masson’s trichrome (l, n, o, p) staining of grafts harvested at POD9 from the mice treated with CD8 mAb with control diet (Cont, e, g, h, k, I, o) or DSF-containing diet (DSF, f, i, j, m, n, p). Low-power views (original magnification ×40, e, f, original magnification ×100 o, p). 8 high-power views (original magnification ×400, g, h, i, j, k, l, m, n). Graft survival was compared using log-rank survival statistics (b). **P  <  0.01, ***P  <  0.001. Significance was calculated using one-way ANOVA with Tukey’s post-hoc test (c): *P < 0.05, ***P < 0.001, ****P   < 0.0001. Data are shown as the mean ± SEM. a was generated using Servier Medical Art, licensed under a Creative Commons Attribution 3.0 Unported License.

Back to article page