Fig. 3: DSF impaired antibody deposition in transplanted grafts and key features of AMR and serum donor-specific antibody level. | Communications Biology

Fig. 3: DSF impaired antibody deposition in transplanted grafts and key features of AMR and serum donor-specific antibody level.

From: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism

Fig. 3

a Immunofluorescence staining image of antibody deposition of IgM (upper line) and IgG (lower line) around the vessel of the grafts (Syn, syngeneic Tx; Cont, allogenic Tx with CD8-depletion and control diet; and DSF, allogenic Tx with CD8-depletion and DSF diet). Quantification of areas positive for IgM (b) and IgG (c) (Syn; syngeneic, 12 fields in three mouse grafts; Cont, 36 fields in four mouse grafts; DSF, 34 fields in four mouse grafts. Plasma donor-specific antibody IgM (d) and IgG (e), (Naïve, n = 3; Cont, n = 17; DSF n = 19 biologically independent mice). IHC staining of F4/80 (upper) and complement C4d (lower) of the grafts (f). Original magnification ×400. Quantification of the F4/80 positive area (upper) and C4d positive area (lower) in control mice (25 fields in three mouse grafts), DSF mice (25 fields in four mouse grafts) and syngeneic mice (15 fields in three mouse grafts) (g). Significance was calculated using a two-tailed, unpaired Student’s t test. **P < 0.01, ***P < 0.001, ****P < 0.0001 Data are shown as mean ± SEM.

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