Fig. 6: Inhibitory effect of DSF on the B cell response was related to the pyrimidine metabolism pathway. | Communications Biology

Fig. 6: Inhibitory effect of DSF on the B cell response was related to the pyrimidine metabolism pathway.

From: Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism

Fig. 6

KEGG pathway (down-regulated genes) enrichment analysis of RNA-seq data of B cells 3 h after stimulation by IL4+anti-CD40 antibody (IL4CD40) (a) or LPS (b), pathways downregulated in DSF-pre-treated B cells compared with those in B cells without DSF. c Hierarchical clustering and heat map of mRNA transcript abundance in B cells unstimulated (NC) or stimulated with IL-4 plus anti-CD40 antibody or LPS with DSF (IL4CD40DSF and LPSDSF) or without inhibitor (IL4CD40 and LPS). Gene expression FPKM values obtained from RNA-seq analysis related to salvage pathway (d) and de novo pathway (e) and in pyrimidine synthesis (n = 4 biologically independent samples). Significance was calculated using one-way ANOVA with Tukey’s post-hoc analysis: *P  <  0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Data are shown as the mean ± SEM.

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