Fig. 3: PCK2 controls channeling of glutamine- and lactate-derived carbons into lower glycolytic intermediates in glucose-deprived ECs.
From: The gluconeogenesis enzyme PCK2 has a non-enzymatic role in proteostasis in endothelial cells
a Heatmap of transcript levels of metabolic genes involved in gluconeogenesis (GNG), glycolysis, pentose phosphate pathway (PPP), serine and glycine biosynthesis pathway, 1-carbon (1 C) metabolism, aspartate and glutamine metabolism and amino acid (AA) transporters (transp.) from bulk RNA sequencing of ECs in 5.5 versus 0 mM glucose (n = 3). Color scale: red, high expression; blue, low expression. b, c Incorporation of [U13C]-glutamine and [U13C]-lactate carbon into total intracellular phosphoenolpyruvate (PEP) pool (b; n = 6) and 2/3-phosphoglycerate (2/3-PG) pool (c; n = 7) in ECs in 5.5 versus 0 mM glucose. d, e Incorporation of [U13C]-glutamine and [U13C]-lactate carbon into total intracellular PEP pool (d) and 2/3-PG pool (e) in control and PCK2KD1 glucose-deprived ECs (n = 5). f Intracellular levels of PEP, normalized (norm.) to protein content (expressed in AUC/µg protein), in control and PCK2KD1 glucose-deprived ECs (n = 3). AUC, area under the curve. g Incorporation of [U13C]-glutamine and [U13C]-lactate carbon into total intracellular dihydroxyacetone phosphate (DHAP) pool in ECs in 5.5 versus 0 mM glucose (n = 3). Data are mean ± s.e.m. Statistics: two-tailed t-test with Welch’s correction (b–g); *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.
