Fig. 3: The inter-subunit disulfide bridge favors the basal activity of mGluRs.
From: Absence of calcium-sensing receptor basal activity due to inter-subunit disulfide bridges
a Schematic representation of the mGluR homodimers. b Structure of mGluR5 VFT (PBD: 7FD8) and the sequence alignment of the upper loop of the human CaSR (Cys 129 and Cys 131 are indicated) and rat mGluRs using Clustal Omega and ESPript 3. CaSR is used as reference for residue numbering and the blue box indicated the most conserved residues. c, e Basal IP1 accumulation for the WT and indicated mutants of mGluR2 (c, n = 4) of mGluR5 (e, n = 5). d, f Intracellular calcium release mediated by the indicated mutants of mGluR2 (d, n = 6) or mGluR5 (f, n = 5) upon stimulation with glutamate and the corresponding pEC50. Data above are mean ± SEM for each individual experiment and normalized to the mock (c, e) or the maximum response of WT (d, f). Significance was analyzed using one-way ANOVA with Dunnett’s multiple comparisons with ****P ≤ 0.0001, ***P ≤ 0.001, **P ≤ 0.01, and *P ≤ 0.05 versus the WT.
