Fig. 4: Genetic mutations at Cys129 and Cys131 favor the constitutive activity of CaSR.
From: Absence of calcium-sensing receptor basal activity due to inter-subunit disulfide bridges
a Scheme showing the reported ADH mutations at Cys129 and Cys131. b Basal IP1 accumulation measured for the WT and indicated mutants (n = 3). c, d Intracellular calcium release in WT and indicated mutants stimulated with CaCl2 and the corresponding pEC50 (n = 3–5). e, f Intracellular calcium release measured for the WT and indicated mutants stimulated by PAM R568 in the absence of ligands (n = 3–5). Data above are mean ± SEM of at least three independent experiments performed in triplicates and normalized to the mock (b) or the WT (c–f). Significance was analyzed using one-way ANOVA with Dunnett’s multiple comparisons with ****P ≤ 0.0001, **P ≤ 0.01, *P ≤ 0.05, and ns for P > 0.05 versus the mock (b) or the WT (c, d).
