Fig. 3: Involvement of CEBPA and FOXA1 in HAL expression. | Communications Biology

Fig. 3: Involvement of CEBPA and FOXA1 in HAL expression.

From: Wnt/β-catenin signaling regulates amino acid metabolism through the suppression of CEBPA and FOXA1 in liver cancer cells

Fig. 3

a Expression of HAL in HuH-7 and Hep3B cells treated with the indicated siRNAs. Expression was assessed by RT-qPCR. The y-axis represents the fold change in the expression of HAL observed in the cells treated with the indicated siRNAs compared to control siRNA. b Expression of HAL, CEBPA, FOXA1, and FOXA3 in the cells treated with the indicated siRNAs was detected by immunoblotting. β-actin served as a loading control. c The effect of CEBPA, FOXA1, and FOXA3 over-expression on the expression of HAL in HepG2 and Hep3B cells. Expression was assessed by RT-qPCR. The y-axis represents fold change in HAL expression observed in the cells over-expressing the indicated transcription factors relative to EGFP (control). d Expression of HAL, CEBPA, FOXA1, and FOXA3 in the cells over-expressing the indicated plasmids was detected by immunoblotting. β-actin served as a loading control. Unless specified otherwise, data are represented as the mean ± SD of three independent experiments. Statistical significance was determined by Dunnett’s test. **p < 0.01 vs siCtrl or EGFP.

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