Fig. 4: Gene set enrichment analysis using genes directly regulated by CEBPA and FOXA1 in liver cancer cells.

a Visualization of ChIP-seq peaks of CEBPA (magenta) and FOXA1 (blue) in the genomic region of HAL. b Venn diagrams depicting the number of genes regulated by CEBPA and genes regulated by FOXA1 in HuH-7 cells. The overlapping genes were considered as direct targets of each transcription factor. c Over-representation analysis (ORA) using KEGG pathway gene sets with the 460 and 489 genes directly regulated by CEBPA and FOXA1, respectively. Significant pathways are shown with q-value and the number of genes. d Venn diagram showing common direct targets of CEBPA and FOXA1. e ORA using the 132 common target genes (FDR q-value < 0.01). f The expression levels of AMD1, ARG1, GLS, and GOT1 in HepG2 cells treated with β-catenin or TCF7L2 siRNA analyzed by RT-qPCR. HPRT1 was used as an internal control. Data are represented as the mean ± SD of three independent experiments. Statistical significance was determined by Dunnett’s test. **p < 0.01 vs siCtrl. g Visualization of ChIP-seq peaks of CEBPA (magenta) and FOXA1 (blue) in the genomic region of ARG1. h Expression of HAL and ARG1 in HepG2 cells treated with CEBPA and/or FOXA1 siRNA in combination with β-catenin siRNA.