Fig. 6: Glucose metabolism in the liver of ADAR2 KO mice.

a Plasma glucose levels during PTT in male mice fed with ND and HFD and analysis of area under the curve (AUC) of IPPTT results. n = 9 mice per group. b Basal and pyruvate-induced glucose release into the medium after 4 h incubation in primary hepatocytes isolated from ADAR2 KO and WT mice. Results are derived from three independent experiments performed in triplicate. c Insulin-stimulated glucose uptake in primary hepatocytes from ADAR2 KO and WT mice. Results are derived from three independent experiments performed in triplicate. d Western blot assays of the proteins associated with gluconeogenesis in each group and Quantitative analysis of the proteins associated with gluconeogenesis in each group (n = 6 mice/group, three independent experiments). e Representative photographs of PAS staining in liver sections. n = 5 mice per group. All data are expressed as mean ± SEM. Tukey’s multiple comparison test after the two-way ANOVA was conducted for (a, b, d and e). Unpaired two-tailed Student’s t-test was conducted for (c). *ND-WT group versus HFD-WT group or ND-KO group versus HFD-KO group; *p < 0.05, ****p < 0.0001; #HFD-WT group versus HFD-KO group; #p < 0.05, ##p < 0.01, ####p < 0.0001; n.s, not significant.