Fig. 3: Determining the impact of inoculation conditions.
a 24 colonies grown from various initial droplet sizes (marked µl drops) and initial cell numbers (labeled initial cell counts) after growth for 14 days. Blue circles indicate the size of the colonies on the second day which approximates the original area occupied by the inoculating droplet. (Scale bar represents 1 cm). b Simulation of the model with a similar layout. Blue circles indicate the area after 2 days. c Area growth curves of the experiment (markers of 4 replicate plates) and fitted simulations (solid lines). Dashed line at 100 mm2 was inserted for easier comparison. Simulation results are scaled 55.6 pixels to 1 mm2.
