Fig. 5: Colony growth in uneven environment.
a Colonies were grown on OmniTray plates with even (left) or uneven (middle and right) nutrient supply for 20 days. Inoculation happened on the lower part (80% of the area) of the plate only, while nutrient was supplied on the top part (20% of the area) in the two plates on the right. Nutrient supply is indicated on the left side of the panels: control (first plate) had YPD in the whole plate, on the second plate 20% YPD and 80% only agar was used, while on the third plate, 20% 5-times concentrated YPD and 80% only agar was added. Pictures were taken on day 13 after inoculation. Green circles indicate initial colony sizes, 1−2 days after inoculation and magenta circles indicate area after 20 days, showing in which directions colonies expand between 13 and 20 days. The experiment was repeated twice with 2 plates per condition. Scale bar is 10 mm. b Colony area and shape are shown from the simulations of the layouts above. c Actively dividing cells on day 13 of the simulations, predicting directions of future growth. Agent density is indicated on a color scale.
