Fig. 5: CHAC1 promotes skeletal muscle regeneration. | Communications Biology

Fig. 5: CHAC1 promotes skeletal muscle regeneration.

From: Whole transcriptome profiling reveals a lncMDP1 that regulates myogenesis by adsorbing miR-301a-5p targeting CHAC1

Fig. 5

a H&E staining of chicken GAS muscle sections at days 1, 3, 5, and 7 after BaCL2 injury following pAV-CHAC1-3xFLAG injection, pAV-3xFLAG as control, scale bar 50 μm. b Quantification of GAS fiber diameter and (c) muscle fiber CSA, n = 3 biologically independent samples. d mRNA expression levels of CHAC1, n = 3 biologically independent samples. e–g mRNA expression levels of muscle regeneration marker genes aMyHC, eMyHC, and Desmin, n = 3 biologically independent samples. h Protein expression levels of CHAC1 and Desmin, n = 3 biologically independent samples. Western blot was performed on different membranes for proteins with a molecular weight difference of fewer than 5 kDa (similar protein size, difficult to distinguish), and the sample size was consistent under the premise of detecting protein concentration. In this case, the sample and the loading control ran on different gels and thus transferred to different membranes. The results are shown as the mean ± SEM of three independent experiments. (*P < 0.05; **P < 0.01, ***P < 0.001).

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