Fig. 6: LncMDP1 acts as a miR-301a-5p sponge to attenuate its inhibitory of CHAC1.

a–d The mRNA and protein expression levels of CHAC1 and proliferation and differentiation marker genes after cotransfection with si-lncMDP1, si-NC, miR-301a-5p mimic, and mimic NC, n = 3 biologically independent samples. The error bars are equivalent throughout the Figure. In the box line, a line in the middle of the box represents the median, and the upper and lower bottoms of the box are the upper quartile and the lower quartile, respectively. The blue circle represents si-NC and mimic NC, the yellow circle represents si-lncMDP1 and mimic NC, the pink circle represents si-NC and miR-301a-5p mimic, and the orange circle represents si-lncMDP1 and miR-301a-5p mimic. Western blot was performed on different membranes for proteins with a molecular weight difference of fewer than 5 kDa (similar protein size, difficult to distinguish), and the sample size was consistent under the premise of detecting protein concentration. In this case, the sample and the loading control ran on different gels and thus transferred to different membranes. e–h The mRNA and protein expression levels of CHAC1 and proliferation and differentiation marker genes after cotransfection with si-lncMDP1, si-NC, miR-301a-5p inhibitor, and inhibitor NC, n = 3 biologically independent samples. The results are shown as the mean ± SEM of three independent experiments. The error bars are equivalent throughout the Figure. In the box line, a line in the middle of the box represents the median, and the upper and lower bottoms of the box are the upper quartile and the lower quartile, respectively. The blue circle represents si-NC and inhibitor NC, the yellow circle represents si-lncMDP1 and inhibitor NC, and the pink circle represents si-lncMDP1 and miR-301a-5p inhibitor. Western blot was performed on different membranes for proteins with a molecular weight difference of fewer than 5 kDa (similar protein size, difficult to distinguish), and the sample size was consistent under the premise of detecting protein concentration. In this case, the sample and the loading control ran on different gels and thus transferred to different membranes. (*P < 0.05; **P < 0.01, ***P < 0.001).