Table 1 Primers used in this study
From: Aging-regulated PNUTS maintains endothelial barrier function via SEMA3B suppression
Use | Gene | Forward primer (5ʹ-3ʹ) | Reverse primer (5ʹ-3ʹ) |
|---|---|---|---|
qPCR | Human PNUTS | GGGTTCGGGTCCCATAGACCCC | GCCGCCAACGTCAATAAATTTGAC |
Human p21 | AGTCAGTTCCTTGTGGAGCC | CATTAGCGCATCACAGTCGC | |
Human RPSA | ATAAGGACGTCATTTCCTGCC | TTTAAGTTACGACGGGAATCCAGAA | |
Human RPLP0 | TCGACAATGGCAGCATCTAC | ATCCGTCTCCACAGACAAGG | |
Human SEMA3B | TGTGCTCCGGAGACTCGT | CGCTGGAAAGTCCACTCCAC | |
Mouse Pnuts | TGGGCAGTGGACATCGTTCTCA | GGCGGTTTGACATGTCTCCTCCA | |
Mouse Sema3b | CACCTCCAGTGGTGTCTTCC | AAAGGTCCCAAGAAGGCTCG | |
Mouse Rplp0 | GCGTCCTGGCATTGTCTGT | GAAGGCCTTGACCTTTTCAGTAA | |
Mutagenesis | PNUTS | TGGATGAAACAGAGCGGGTAAATGTGA | TCACATTTACCCGCTCCGTTTCATCCA |
Genotyping | Cre | GCGGTCTGGCAGTAAAAACTATC | GTGAAACAGCATTGCTGTCACTT |
Cpxm1 | ACTGGGATCTTCGAACTCTTTGGAC | GATGTTGGGGCACTGCTCATTCACC | |
PNUTSfl | CATGACAATATGGAGGAGAAAGTGCCTTG | AGGAGAAAGAAGAGAACAGGGGACCATAAT |
