Fig. 7: AZ’6421 degrades ERα under hypoxic conditions in vitro.
From: Metabolism-driven in vitro/in vivo disconnect of an oral ERɑ VHL-PROTAC
a Immunoblots for HIF1α and GAPDH following incubation of MCF7 cells at 2% O2 within a Tri-gas incubator to induce hypoxia. b Effect of PROTAC and fulvestrant treatment on ERα degradation under normoxic and hypoxic conditions. MCF7 cells were exposed to normoxic or hypoxic conditions and treated with compound or 0.1% DMSO vehicle control for 48 h prior to being lysed. c ERα levels were normalised to vinculin and expressed as a percentage (%) of vehicle control. The data shown is representative of two separate experiments.
