Fig. 3: O2•− fluorescence imaging of LX-2 cells. | Communications Biology

Fig. 3: O2•− fluorescence imaging of LX-2 cells.

From: Fluorescence imaging sheds light on the immune evasion mechanisms of hepatic stellate cells mediated by superoxide anion

Fig. 3

a O2•− fluorescence imaging of endogenous O2•− in LX-2 cells. a1: control; a2: 2-Me (0.1 µg mL−1) for 15 min; a3: after 2-Me incubation, Tiron (10 µM) was added and incubated for 30 min. c O2•− fluorescence imaging of LX-2 cell activation. c1: control; c2: TGF-β1 (5 ng mL−1) was incubated for 12 h; c3: TGF-β1 was incubated and Tiron (10 µM) was added for 30 min. b, d Fluorescence intensities of (a) and (c). Scale bar = 50 μm (a) or 10 μm (c). The data were expressed as mean ± SD, n = 3. *p < 0.01, **p < 0.05, ***p < 0.001 compared to the control group.

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