Fig. 2: Flanking sequence preference analysis of DNMT3 enzymes. | Communications Biology

Fig. 2: Flanking sequence preference analysis of DNMT3 enzymes.

From: Specific DNMT3C flanking sequence preferences facilitate methylation of young murine retrotransposons

Fig. 2

a Schematic picture of the design of the random flank substrates. b-e Combined -4 to +4 flanking profiles of WT DNMT3C, WT DNMT3B, WT DNMT3A and the DNMT3C C543N/V547A mutant. DNMT3A data were combined from19,29; DNMT3B data were from19. Shown are observed/expected (o/e) values for the occurrence of individual bases at each position in the methylated products. DNMT3C-specific C-preference at position -2 and -1 is highlighted by blue arrows, the DNMT3A and DNMT3B characteristic preference for T(-2) is labelled with a red arrow. See also Supplementary Figs. 2 and 3. f Correlation of the combined -8 to +8 flank profiles of the different DNMT3s. Shown are Pearson r values.

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