Fig. 1: In vitro interaction between SIB1 and WRKY33_C.
From: Structural basis for the regulation of plant transcription factor WRKY33 by the VQ protein SIB1
a An illustration of the soluble SIB111-100 truncation construct. b Gel electrophoresis of the binding between W-box DNA and WRKY33_C under different molar ratios in the presence or absence of SIB1 (left) and the band intensity read-outs corresponding to binary or ternary complexes (right). WRKY33_C is designated as WK33 for short. c SDS-PAGE analysis of the chemical cross-linking results between WRKY33_C and SIB1. The bands corresponding to the free WRKY33_C, SIB1, and the cross-linked complex are indicated by red arrows (note that both SIB1 and WRKY33_C migrate at apparent molecular weights much larger than their theoretical values). d Size-exclusion chromatography profile showing the complex formation between WRKY33_C and Trx-SIB1. The profile of the mixed sample of Trx_SIB1 and WRKY33_C is shown in red, and that of protein standards for molecular weight calibration is shown in black.
