Fig. 3: Identification of SIB1-WRKY33_C binding sites.
From: Structural basis for the regulation of plant transcription factor WRKY33 by the VQ protein SIB1
a Schematic illustration of a series of SIB1-derived peptides used to identify the minimal sequecne required for WRKY33_C interaction. The sequence corresponding to SIB1mini is colored in red, and the consensus VQ motif is highlighted. b The 1H-15N HSQC spectra of WRKY33_C in the free state (black) and in complex with SIB1 (blue) and SIB1mini (magenta). c The CSP profiles of the 15N-labeled WRKY33_C upon titration of SIB1 or SIB1mini. Gray bars indicate residues that are missing the complexed state. d Structural model of WRKY33_C shown as the cartoon diagram (left) and the surface representation showing the charge distribution (right). e Mapping of the obviously perturbed residues onto the WRKY33_C surface representation.
