Fig. 2: A schematic pipeline for network reconstruction.

a Workflows begin with the acquisition of neuron images through microscopy. b Pre-processing techniques aim to improve image signal-to-noise ratio and reduce ambiguities. c Segmentation creates a mask of neuron morphology (top left of panel), which can be skeletonized (bottom left of panel). Tracing creates a tree of neuron centrelines (right half of panel). d Morphological labelling resolves the neuron mask into features such as somata (orange) and neurites (yellow). e Post-processing methods refine or extract features, such as branch points (blue) from the neuron skeleton (yellow). f Network reconstruction creates a model representing morphological features as nodes and their relationships as edges.