Fig. 4: Distinct functional signatures of the melanoma tumor cells. | Communications Biology

Fig. 4: Distinct functional signatures of the melanoma tumor cells.

From: Zebularine potentiates anti-tumor immunity by inducing tumor immunogenicity and improving antigen processing through cGAS-STING pathway

Fig. 4

a T-distributed stochastic neighbor embedding (t-SNE) plot, showing the annotation and color codes for mock and zebularine-treated B16F10 melanoma and their integration. b Heat map showing the expression score by ssGESA in the 7 clusters, including related signal pathways. c, d Histograms represent the proportion of cell cycles and the expression levels of related genes in B16F10 after zebularine treatment. e-f B16F10 tumor-bearing wild-type mice were treated with zebularine. After 12 days, tumor tissue was collected. Immunofluorescence images showing proliferation of B16F10 tumor tissues, based on Mki67 (e) and Pcna (f), in the mock and zebularine groups. Data are presented as mean ± SEM of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t-test.

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