Fig. 5: Single cell analysis identified intracatumoral cell populations and confirmed the association of zebularine in tumor innate signaling and immunity. | Communications Biology

Fig. 5: Single cell analysis identified intracatumoral cell populations and confirmed the association of zebularine in tumor innate signaling and immunity.

From: Zebularine potentiates anti-tumor immunity by inducing tumor immunogenicity and improving antigen processing through cGAS-STING pathway

Fig. 5

a Volcano plot of single-cell RNA-seq data shows differentially expressed genes (DEGs) in mock (blue dots) and zeb (red dots) group. The most significant DEGs are indicated on the plot. b Gene Ontology (GO) shows the enrichment of upregulated genes. c KEGG pathway analysis shows the pathways of the enriched by upregulated genes. d Violin diagram showing the expression of Mock (blue) and zebularine treated (red) clusters. e, f mRNA levels of indicated genes from B16F10 (e) and MC38 (f) cells treated with DMSO or zebularine for 72 h measured by RT-qPCR. g mRNA levels of indicated genes from B16F10 tumor-bearing mice treated with PBS or zebularine for 12 days, measured by RT-qPCR. Data are presented as mean ± SEM of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t-test.

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