Fig. 6: DNMTi upregulates antigen processing and presentation.

a Violin diagram showing the expression of Mock (blue) and zebularine treated group (red) clusters. b, c mRNA levels of indicated genes from B16F10 (b) and MC38 (c) cells treated with DMSO or zebularine for 72 h measured by RT-qPCR. d mRNA levels of indicated genes from A375 cells treated with DMSO or zebularine for 7 days measured by RT-qPCR. e mRNA levels of indicated genes from B16F10 tumor-bearing mice treated with PBS or zebularine for 12 days measured by RT-qPCR. f–i Expression of H-2Kb/H-2Db on the surface of B16F10 (f, g) and MC38 (h, i) cells after 72 h of zebularine treatment, detected by flow cytometry. j Expression of HLA-A/B/C on the surface of A375 cells after 7 days of zebularine treatment, detected by flow cytometry. k–l B16F10 cells were transfected with control siRNA (NC) or siRNA targeting Dnmt1 (318 and 1387) for 3 days. RT-qPCR was used to detect the expression of Dnmt1 (k) and flow cytometry was used to detect the expression of H-2Kb/H-2Db on the surface of B16F10 cells (l). Data are presented as mean ± SEM of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t-test.