Fig. 7: Zebularine up-regulated antigen processing and presentation dependent on cGAS-STING-NF-κB/IFNβ signaling. | Communications Biology

Fig. 7: Zebularine up-regulated antigen processing and presentation dependent on cGAS-STING-NF-κB/IFNβ signaling.

From: Zebularine potentiates anti-tumor immunity by inducing tumor immunogenicity and improving antigen processing through cGAS-STING pathway

Fig. 7

a RT-qPCR and immunoblot showing the effect of shRNA Real/p65 knockdown in B16F10 cells. b Surface expression of H-2Kb in Rela/p65 knockdown B16F10 cells 72 h after zebularine treatment, detected by flow cytometry. c shRNA-Ctrl or Rela/p65-silenced B16F10 cells were incubated with zebularine as indicated. mRNA levels of indicated gene measured by RT-qPCR. d Surface expression of MHC-I (H-2Kb and H-2Db) in B16F10 tumor cells treated with increasing doses of BMS-345541, a selective NF-κB inhibitor, detected by flow cytometry. e, f Ctrl, CRISPR-Cas9, or shRNA-silenced B16F10 cells were treated zebularine and surface MHC-I (H-2Kb and H-2Db) expression measured by flow cytometry. g mRNA levels of indicated genes from B16F10 tumor-bearing wild-type and IFNAR1-/- mice treated with PBS or zebularine for 12 days, measured by RT-qPCR. Data are presented as mean ± SEM of at least three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001 by Student’s t-test.

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