Fig. 2: Exogenous lipases enable cholesterol-dependent growth in the presence of AFAs.
From: Lipase-mediated detoxification of host-derived antimicrobial fatty acids by Staphylococcus aureus
a Wild-type USA300 JE2 and its isogenic Δlip mutant with pEmpty, and Δlip complemented with plip2 were grown in plain nutrient broth (NB), or NB with or without USA300 membrane vesicles (MVs) and supplemented with 50 µM palmitoleic acid (PA) or 50 µM PA + 50 µM cholesterol (Chol). Computed area under growth curves was plotted. b Area under the curves of the strains described in (a) upon growth in NB, or NB supplemented with 50 µM sapienic acid (SA) or 50 µM SA + 50 µM Chol, with or without recombinant Lip2. Data shown are means plus SEM (n = 3). Statistical significance was evaluated by two-way ANOVA with Tukey’s multiple comparisons test. **p = 0.0019, ***p = 0.0009, ****p < 0.0001.
