Fig. 3: Catalytically active Lip2 is required for cholesterol-mediated resistance to AFAs.
From: Lipase-mediated detoxification of host-derived antimicrobial fatty acids by Staphylococcus aureus
Wild-type USA300 JE2 and its isogenic Δlip mutant with pEmpty, and Δlip complemented with either plip1 or plip2 were grown for 24 h in (a) basic medium (BM), or BM supplemented with (b) 100 µM palmitoleic acid (PA), (c) 100 µM cholesterol (Chol), or (d) 100 µM PA + 100 µM Chol. Cultures were then serially diluted and spotted onto TSB plates. Dilution factors are indicated below spots. One representative biological replicate (n = 3) is shown. e Area under the curves of pEmpty-bearing wild-type USA300 JE2 and its Δlip mutant, and Δlip complemented with either plip2S412A or plip2 cultured for 24 h in BM, or BM supplemented with 100 µM PA or 100 µM PA + 100 µM Chol. Data shown are means plus SEM (n = 3 or 4). Statistical significance was evaluated by one-way ANOVA with Tukey’s multiple comparisons test. ****p < 0.0001.
