Table 1 Strains and plasmids
From: Lipase-mediated detoxification of host-derived antimicrobial fatty acids by Staphylococcus aureus
Strain or plasmid | Description | Source or reference |
|---|---|---|
Staphylococcus aureus | ||
Newman | Methicillin-sensitive S. aureus (MSSA) | |
SH1000 | NCTC8325 derivative with a functional rsbU gene, ΔtcaR, cured of φ11, φ12, and φ13; MSSA | Simon Foster84 |
USA300 | Community-acquired MRSA (CA-MRSA), plasmid-cured derivative of LAC strain | David E. Heinrichs23 |
USA300 Δlip2 | USA300 with the lip2 (gehB) gene deleted | David E. Heinrichs23 |
USA300 JE2 | CA-MRSA, plasmid-cured derivative of LAC strain | Paul Fey85 |
USA300 JE2 Δlip | USA300 JE2 defective for Lip1 and Lip2 | Friedrich Götz21 |
USA400 MW2 | CA-MRSA | Michael Otto86 |
USA400 MW2 Δlip1 | MW2 with the lip1 (gehA) gene deleted | This study |
USA400 MW2 Δlip2 | MW2 with the lip2 (gehB) gene deleted | This study |
Escherichia coli | ||
BL21 (DE3) | F– ompT hsdSB(rB– mB–) gal dcm (DE3), IPTG-inducible T7 RNA polymerase | New England Biolabs |
DC10B | Δdcm in the DH10B background (K-12 derivative) | Simon Heilbronner69 |
SA08B | DC10BΩPhelp-hsdMS (CC8-2) of NRS384 integrated between the atpI and gidB genes | Simon Heilbronner87 |
IM01B | SA08BΩPN25-hsdS (CC1-1) of MW2 integrated between the essQ and cspB genes | Simon Heilbronner87 |
Plasmid | ||
pIMAY | Temperature-sensitive allelic exchange plasmid for staphylococci | Simon Heilbronner69 |
pIMAY-Δlip1 | pIMAY carrying the lip1(gehA) deletion construct. | This study |
pIMAY-Δlip2 | pIMAY carrying the lip2 (gehB) deletion construct. | This study |
pEmpty (pALC2073) | E. coli-S. aureus shuttle vector; contains Pxyl/tet | David E. Heinrichs70 |
plip1 | pALC2073 containing the lip1 coding region | This study |
plip2 | pALC2073 containing the lip2 coding region | David E. Heinrichs23 |
plip2S412A | pALC2073 containing the mutated lip2 coding region to generate Lip2 S412A | This study |
pET28a(+)-lip2 | pET28a(+) for Lip2 expression | David E. Heinrichs23 |
pET28a(+)-lip2S412A | pET28a(+) for Lip2 S412A expression | David E. Heinrichs23 |
