Table 2 Primers
From: Lipase-mediated detoxification of host-derived antimicrobial fatty acids by Staphylococcus aureus
Oligonucleotide | Sequence (5’—3’) |
|---|---|
For deletion of lip1 (pIMAY-Δlip1): | |
KpnI-lip1 ups-fwd | CTAGAAGGTACCGGTGTCGGCATGATATTGCG |
lip1 ups-rev | CAAGCATAATTTATAAAGTAAAGGGAGG |
SOEING-lip1 dwn-fwd | CCTCCCTTTACTTTATAAATTATGCTTGACTTTTCATCATTGTCAGCACCTC |
SacI-lip1 dwns-rev | GCATCCGAGCTCGTAGGATACTTACTTTGAGGGAAG |
For deletion of lip2 (pIMAY-Δlip2) | |
KpnI-lip2 ups-fwd | GTAGAGGTACCGTATGCCCACTAAACTATAGAC |
lip2 ups-rev | TCCTCTTAACATATAATCACCTC |
SOEING-lip2 dwn-fwd | GAGGTGATTATATGTTAAGAGGAGCAAGTTAAATTCATCTTCTG |
SacI-lip2 dwns-rev | CATGTTGAGCTCCGTTTATGCACGTGGCACAGG |
For site-directed mutagenesis of plip2 into plip2S412A | |
lip2S412A-fwd | GTAGGGCATGCTATGGGTGG |
lip2S412A-rev | CAAGATGTACCTTTTTACCAGG |
