Fig. 4: Overexpression of HNF4A in dHepaRG cells represses basal acute-phase gene expression.
From: Downregulation of HNF4A enables transcriptomic reprogramming during the hepatic acute-phase response
a Generation of stable cell lines for DOX-inducible overexpression of myc-tagged HNF4A. b Whole cell protein extracts showing levels of endogenous and myc-tagged HNF4A after 48 h of DOX treatment in HepG2 and HepaRG cell lines. n = 3. c Immunofluorescence images of HNF4A (green) in HepaRG HNF4A_myc cell line under untreated and doxycycline-treated (400 ng/mL, 48 h) conditions, scale bar = 100 µm. d DOX-dependent increase in expression of HNF4A_myc, endogenous HNF4A P1, HNF1A and G6PC1. e DOX-dependent decrease in acute-phase genes HP, SAA1/2 and FGG in dHepaRG HNF4A_myc and ev cells after 48 h of DOX (25; 100; 400 ng/mL) treatment. Data shown in (d, e) represent as mean ± SEM from n = 3 independent experiments, one-way ANOVA post-hoc Holm-Sidak test, significance levels *< 0.05; **< 0.01; ***< 0.001; ****< 0.0001).
