Fig. 6: HNF4A overexpression in a microfluidically perfused liver organoid preserves liver functionality during LPS-induced damage.
From: Downregulation of HNF4A enables transcriptomic reprogramming during the hepatic acute-phase response
a Scheme for seeding, cultivation and treatment with DOX (400 ng/mL, 48 h) and LPS (200 ng/mL, 24 h) of the liver-on-chip organoid system. Created with Biorender.com. b–d Expression analysis via RT-qPCR of whole liver-on-chip RNA for (b) cytokines and chemokines, (c) HNF4A_myc and respective targets of liver homeostatic function and (d) acute phase genes (n = 3, 2-way ANOVA post-hoc Holm-Sidak test). e Immunofluorescence of the hepatic layer from the liver-on-chip after the indicated treatments with DOX/LPS, white box indicates highly differentiated hepatocyte clusters, scale = 100 µm. f, g Measurements of LDH (f) and ASAT (g) concentrations in hepatic supernatant (n = 3, 2-way ANOVA post-hoc Holm-Sidak test). Data are expressed as mean ± SEM, significance levels *< 0.05; **< 0.01; ***< 0.001; ****< 0.0001.
