Fig. 5: In suitable alleles, insertion sequences can enhance their peptide binding capacity and promote tolerance to high temperatures.
From: Amino acid insertion in Bat MHC-I enhances complex stability and augments peptide presentation

a Thermal stabilities of pMylu-B*67:01-R9Ps and pMylu-B*67:01ΔMQQPW-R9Ps as shown by CD results. b The MQQPW insertion enhanced the tolerance of pMylu-B*67:01-R9Ps to 41°C. The vertical coordinate is the UV absorption value of the complex peak in the gel filtration chromatogram. c MQQPW insertion significantly increased the number of peptides with different lengths bound by Mylu-B*67:01. The line graph on the right represented the UV absorption value of the complex peak in the gel filtration chromatogram. d Thermal stability of pPtal-N*01:01-R9Ps and pPtal-N*01:01ΔMDL-R9Ps as shown by CD results. e The MDL insertion enhanced the tolerance of pPtal-N*01:01-R9Ps to 41°C. f MDL insertion significantly increased the number of peptides with different lengths bound by Ptal-N*01:01. The line graph on the right represented the UV absorption value of the complex peak in the gel filtration chromatogram. g Differential effects of MDL and MQQPW on the binding capacity of the insertion-free bat MHC-I molecule Mylu-B*57:01 to R9Ps. h Differential effects of MDL and MQQPW on the thermal stabilities of the insertion-free pMylu-B*57:01-R9Ps.