Fig. 6: In CSSLs, the suppression of seed size by DME is inherited across generations.

a Schematic diagram of the CSSL population construction procedure. b Dry seeds of CSSL progeny: R183, R147, R102 (small), R191, R178, and R84 (large).Bar = 1 cm. c 100-seed weight of CSSL progeny:R183, R147, R102 (small, with green bar), R191, R178, and R84 (large, with red bar). d Snapshots from the Integrative Genomics Viewer (IGV) showing DNA methylation and gene expression levels in SAMBA (Glyma.01g015700) and GoLS1 (Glyma.20G094500) in dry seeds of SN14 and ZYD6. e Correlation of 100-seed weight with the expression levels of GmDMEa, SAMBA and GoLS1 in progeny of CSSLs. The green dots represent small-sized seeds, while the red dots represent large-sized seeds. A total of six samples were analyzed, with three biological replicates for each sample.Pearson correlation test; n = 18.