Fig. 6: Measuring neuronal-cell activity.
From: The role of elasticity on adhesion and clustering of neurons on soft surfaces
We used fMCI (functional multicalcium imaging) to measure the activity of neuronal cells on soft PDMS substrates. In the technique, calcium ions within neuronal cell networks are selectively targeted with a fluorescent compound - its transients (a, b) are then associated to the generation and release of action potentials in the system. Intensity of calcium-related fluorescence vs time measured at \(6\) different sites (neurons) of neuronal networks cultured on substrates with decreasing values of elasticity: \(0.55\), \(1\) and \(2.65\) \({{{{{\rm{MPa}}}}}}\) (c). Raster-plot of fluorescence intensity signals shown in c, d. Density of peaks of the fluorescence-intensity signals measured in neuronal-networks as a function of substrate elasticity (e). Data in Fig. 6e are represented by mean ± standard deviation (sample size = 12).
