Fig. 7: Effect of Mito-ortho-HNK on l-dopa/dopamine metabolism in vivo. | Communications Biology

Fig. 7: Effect of Mito-ortho-HNK on l-dopa/dopamine metabolism in vivo.

From: Mitigating gut microbial degradation of levodopa and enhancing brain dopamine: Implications in Parkinson’s disease

Fig. 7

Mice were orally treated with 150 mg/kg l-dopa-d3 with or without Mito-ortho-HNK for 2 h. The effects of Mito-ortho-HNK on dopamine-d3 formation and l-dopa-d3 consumption were measured. l-dopa-d3 and dopamine-d3 were separated and monitored by LC-MS-SIM. Standards are shown in (a). Effects of Mito-ortho-HNK on l-dopa-d3 consumption and dopamine-d3 formation in the mice gut samples were measured, and the representative LC-MS-SIM trace are shown in (b). The formation of dopamine-d3 in mice brain samples was measured as described in the Methods section. c The amount of dopamine-d3 in the brain is based on the area under the curve of the LC-MS-SIM dopamine-d3 peak. Statistical significance was determined using a Student’s t test. The values shown are the fold change in Mito-ortho-HNK-treated mice compared with controls. (**) Statistically significant increase in dopamine-d3 formation compared with control (fold change>15 and P < 0.01). The representative LC-MS-SIM traces are shown in (d).

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