Fig. 3: Activated Th cells enhanced sphingolipid biosynthesis pathway among lipid biosynthesis.

a, Heatmap depict the expression levels of protein related to lipid metabolism. b, PCA depicts profiles of protein related to lipid metabolism in naïve CD4+, Th0, Th1, Th2, Th17, and iTregs cells. c, Venn diagram showed overlaps and differences between 2.0-fold increased proteins related to lipid metabolism in Th0, Th1, Th2, Th17, or iTregs cells relative to naive CD4+T cells. d and e, The lipidomics analysis shows the relative contents of lipid species related to ceramide lipids (d) or glycosphingolipid (e) in Th0, Th1, Th2, Th17, and iTreg cells compared to naïve CD4+ T cells. Four biological replicate was prepared for metabolomics and proteomics analysis. The source data for the figures is provided in Supplementary Data 1 and Supplementary Data 2.